402 Dilutor Dispenser User’s Guide
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November
402 Dilutor Dispenser User’s Guide
LT801310/C - 2003 Gilson SAS All rights reserved
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3 Installation
Table of Contents
Safety
1 Introduction
Operation - Advanced Use
Maintenance
Operation - Standard Use
Table of Contents-2
Index
7 Troubleshooting
Appendices
402 Dual Syringe with Tee Junction Config
Page
Safety
Safety-1
Safety
Introduction1
Notational Conventions
Notational Conventions
Introduction
Introduction
Overview
Overview
Overview
checking protocol for the hydraulic specifica- tions IQ/OQ procedures
Introduction
Good Laboratory Practice GLP is facilitated by the following
Introduction
Unpacking
Unpacking
Introduction
Warranty
Warranty
Introduction
Customer Service
Customer Service
Introduction
Description2
Front Panel
Single-syringeModel
Description2
Rear Panel
Dual-syringe Model
Dual-syringe Model
Description
Front Panel
Probe
Keypad and Probe
Keypad
Keypad and Probe
Tee Junction
Hydraulic Components
Valve
Syringes
There are seven syringe sizes available for the
Description2
Hydraulic Components
Installation3
For Standard Use
Installation
Assembling the Probe
Adapter Kit for Disposable Tips
2 Special adapters for the probe 1 D200 tips, pack of
Installation
Assembling the Probe
Single-syringe Model
Connecting the Tubing
Connecting the Tubing
Valve Inlet Tubing
Tubing
Connecting the
Dual-syringe Model
Valve Waste Tubing
1 mL, 5 mL, and 10 mL are available as additional accessories
Installation
Connecting the Tubing
Sector Block
Installing the Fuses
Electrical Connections
Voltage Selector
Connecting the Probe
Connecting the Power Cord
Connecting the Keypad
Installation
Fitting a Syringe
Syringes
Assembling a Syringe
Installation
Installation
Upgrading to Dual-syringe Model
Upgrading to Dual-syringe Model
Upgrading to Dual-syringe Model
Installation Removing the Cover
Connecting the Flat Cable
3 Secure the module in place at the top using the two screws provided
Inserting the Module
Installation
Upgrading to Dual-syringe Model
Operation - Standard Use
Operation - Standard Use
Fit the Fuses and Connect the Power
Essential Steps
Standard Parts
Start-up Screen
Switch-on
Initialization Screen Initialization Screen Homing Screen
Operation - Standard Use
Configuration Screen Tubing Volume Screen Air Gap Volume Screen
Configuration Conf
Volumes
Operation - Standard Use
Flow Rates
Configuration Screen
Syringes
Operation - Standard Use
Operation - Standard Use
Other Items
Resv Flow Screen Prime Flow Screen Beeper Screen Autorefill Screen
Essential Steps
Creating a Method Edit
Configuration Screen Syringe Screen Counter Screen
Fit the Syringes
Introduction
Start-up Screen Task Selection Screen
In-run Prompts
Method Example
Entering the Method
Dispense Screen 4b Dispense Screen Dispense Screen Dispense Screen
Press ESC, Dispense Screen 7 appears
Dispense Screen Dispense Screen Dispense Screen Dispense Screen 4a
Task Selection Screen Rinse Screen Task Edit Screen Save Screen
Pressing STOP
Prime the 402 Man
Pressing End
Manipulation Screen Initialization Screen Abort Screen
Operation - Standard Use
Running a Method Run
Run Screen Run Screen Abort Screen
Essential Steps
Essential Steps
In Run Screen In Run Screen 2a In Run Screen 2b
Operation - Standard Use
Method Example
Operation - Standard Use
In Run Screen 3b In Run Screen 4b In Run Screen 5b In Run Screen 6b
Abort Screen
Essential Steps
Operation - Standard Use
Essential Steps
Operation - Advanced Use
Correct Use of the
Correct Use of a
Operation - Advanced Use
Liquids should be separated by an air gap to minimize contamination
Operation - Advanced Use
Left-hand Syringe Aspirating from Reservoir
Left-hand Syringe Dispensing from Reservoir
Correct Use of the
Right-hand Syringe Dispensing to
Aspirating Large Volumes via the Probe
Aspirating Small Volumes via the Probe
Probe
Operation - Advanced Use
Software
Software
Volumes
Entering the Configuration CONF
Tubing Volume Screen
Configuration Screen
Operation - Advanced Use
Syringe Screen
Change Size
Software
Correction Factor
Syringe Correction Screen
Air Flow Screen Resv Flow Screen Prime Flow Screen
Operation - Advanced Use
Counters
return to the Configuration Screen
Creating a Method EDIT
press Count, the Syringe Counter Screen appears
Delete a File
Saving a Method
File Manipulations FILE
Syringe Correction Screen Save Screen File Menu Screen
Lock or Unlock a File
Copy a File
File Directory
New File Screen
Locked File Screen Action Screen Initialization Screen Abort Screen
Direct Manipulations MAN
Prime
Operation - Advanced Use
Operation - Advanced Use
Measure
Measure Screen Flow Rate Screen
Software
Measurement Screen
Procedure
Titrate
Operation - Advanced Use
Operation - Advanced Use
Tasks - Detailed Descriptions
Titrate Screen Titrate Screen Titrate Screen Titrate Screen
Software
Operation - Advanced Use
Keys
Parameters
Software
Software
Chaining Tasks
Operation - Advanced Use
Parameter
Dilute Normal
Dilute
Screens
Typical Task Entry
REAG
ENTER
Next
Dilute Serially
Typical Task Entry
Operation - Advanced Use
Software
Screens
Operation - Advanced Use
Dilute Unknown
Typical Task Entry
Software
Software
Run Procedure
Operation - Advanced Use
Software
Dispense
Operation - Advanced Use
Typical Task Entry
Operation - Advanced Use
Software
Software
Pipette
Operation - Advanced Use
Key to press
Operation - Advanced Use
Rinse
Extra
Software
Example
Link
What it Does
Operation - Advanced Use
Key to Press
Configuration
Prefabricated Methods
Edit More Link File # 7 ENTER Next File # 8 ENTER
Software
Running a Method RUN
Operation - Advanced Use
In-run Prompts
Software
When you have finished, or wish to change programs, press STOP, to instantly halt the program. Alterna- tively, you can bring the 402 to a halt by switching off the power, in which case you must ensure that the probe end of transfer-tubing is in a suitable waste container before switching on again
Operation - Advanced Use
Pressing STOP
Flow Rates
In Configuration
Additional Information
Additional Information
Additional Information
Flow Rates - Dual-syringe Model
Operation - Advanced Use
Example
Air Mode
Operation - Advanced Use Syringe Correction Function
Use of Disposable Tips
Additional Information
Additional Information
Liquid Mode
Operation - Advanced Use
Cleaning the exterior of the instrument
Maintenance6
The only maintenance operations necessary for the 402 DD are
Cleaning of the valve, Tee, syringe assemblies and the tubings
Cleaning the Exterior
Cleaning
Maintenance
Cleaning the Valve
Cleaning
Cleaning the Tee Junction
Maintenance
1 Pull the axle away from the valve main body
Cleaning
Cleaning the Syringe Assembly
Maintenance
1 Dismount the Syringe assembly from the instrument
Autoclaving
Autoclaving
Autoclaving the Tubing
Maintenance
Replacing the Piston
Changing the Syringe Piston Assembly
Chaning the Syringe Piston Assembly
Maintenance
Changing the Piston Syringes and Piston Noses
Changing the Piston Syringes and Piston Noses
Changing the 100 and 250 µL Piston Noses
Maintenance
Maintenance
Noses
Changing the Piston
Piston
Maintenance
Changing the 500 µL Piston Noses
Changing the Syringe Piston and Piston Noses
Changing the Piston Syringes
Syringe body Changing the 1 mL Piston Noses
Piston Noses
Maintenance
Maintenance
Changing the 25 mL Piston Noses
Changing the 5 mL and 10 mL Piston Noses
Changing the Syringe Piston and Piston Noses
Maintenance
Changing the Piston Syringes and Piston Noses
Troubleshooting7
Mechanical Problems
Mechanical Problems
Troubleshooting
Troubleshooting
Hydraulic Problems
Hydraulic Problems
Troubleshooting
Electrical Problems
Electrical Problems
Parts & Accessories Lists Appendix A
Appendix A-1
Appendix A
Parts & Accessories Lists
402 Single Syringe
402 Single Syringe
Parts & Accessories Lists
402 Dual Syringe with Tee Junction Configuration
402 Dual Syringe with Tee Junction Configuration
Appendix A
Parts & Accessories Lists
AdditionalAccessories
AdditionalAccessories
Appendix A
Parts & Accessories Lists
Spare Parts
Spare Parts
Appendix A
Appendix A-6
Parts & Accessories Lists
Appendix A
Chemical Compatibility Appendix B
Appendix B-1
Appendix B
Chemical Compatibility
Chemical Compatibility
Materials in Contact with Liquids
Technical Data
Appendix C
Maximum Volume
Technical Data
Minimum Volume
Hydraulics
Technical Data
Performance Specifications
Protocol
Appendix C
See OQ Document for procedure and calculations
Junction Tubing
Technical Data
Appendix C
Technical Data
Mechanics
Mechanics
Valve
Environmental
Power Requirements
Size and Weight
Conformity to Norms
Error Messages
Appendix D
Error Messages
Error Messages
Error Messages
Appendix D
Appendix D
Error Messages
Error Messages
Appendix D-3
Appendix D-4
Error Messages
Appendix D
Index
Index-1
Index
Index-2
Index
Index-3
Index
Index-4
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