Operation - Advanced Use

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Additional Information

Gilson D200 tips are used for volumes from 1 µL to 78 µL (with a 100 µL syringe, minimum recommended air-push-volume is 20 µL). Gilson D1000 tips are used for volumes from 50 µL to 900 µL (minimum recommended air-push-volume is 100 µL).

The liquid is ejected with an Air push volume of at least 20 µL when using a Gilson D200 tip and 100 µL when using a Gilson D1000 tip. An automatic software correction of 2% of the piston stroke

is made to improve accuracy with Gilson D200 and D1000 tips. For example, when using a Gilson D200 tip, if an Air push volume of 20 µL is chosen, the maximum sample volume will be 78 µL; this volume will be handled with the 100 µL syringe. For the dual syringe model, the left syringe operates if the volume exceeds 78 µL; for the single syringe model, an error message is displayed.

A disposable tip should not be fitted, when priming the 402 in ‘Air Mode’. Droplets of liquid adhering the tubing after purging must be wiped from the end, before fitting a tip.

To minimize carry-over, dilution can be performed with one or more tips. If one tip is used, you use the Dilute task where the diluent and the sample are separated by an air gap (volume defined in the Configuration menu). When several tips are used, you use multiple runs of the Dispense task to transfer the liquids, changing the tips where appro- priate: one to aspirate and dispense the diluent, one to aspirate and dispense each sample.

Liquid Mode

You can use this mode in the same way as the standard tip with tapered tubing. However, it is recommended not to use an air gap, or at least to use it in a way that keeps the air gap in the disposable- tip. The use of Gilson D200 tips does not adversely affect the performance of the 402.

The Gilson D200 tip is installed to protect the end of the probe against corrosive chemicals, and by changing the tips within a task.

A disposable tip should be fitted, when priming the 402 in ‘Liquid Mode’. The following flow rates are suggested when priming: small syringes up to 10 mL/ min, larger syringes at least 80 mL/min.

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Gilson 402 manual Liquid Mode
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402 specifications

The Gilson 402 is a highly regarded laboratory device known for its precision and reliability in liquid handling applications. Primarily utilized in research and clinical labs, this automated pipetting system significantly enhances productivity by reducing the time taken for repetitive tasks while ensuring maximum accuracy.

One of the standout features of the Gilson 402 is its intuitive user interface. The touchscreen display allows operators to easily navigate through various functions and settings, enabling quick setup and adjustments. Its intuitive design minimizes the learning curve for new users, making it accessible even for those with limited technical expertise.

The Gilson 402 employs advanced pipetting technologies, which provide exceptional reproducibility and reliability. The system is equipped with proprietary Pipette G (as used in Gilson's PIPETMAN series), which ensures precise volume delivery. This is crucial in applications such as molecular biology, where even minor discrepancies in liquid volumes can lead to significant variations in experimental outcomes.

Another key characteristic of the Gilson 402 is its versatility. The device accommodates a wide range of pipette tips, allowing users to work with varying volumes and types of samples. Whether handling delicate biological fluids or viscous solutions, the Gilson 402 adapts to different protocols seamlessly.

The automation capabilities extend to a customizable programming feature that permits users to create complex pipetting protocols with ease. Multiple-step pipetting tasks can be completed effortlessly, reducing the likelihood of errors caused by manual handling.

Equipped with high-precision motors and sensors, the Gilson 402 also features built-in calibration and quality control functions. This guarantees that the system remains accurate over time and can easily be adjusted to maintain compliance with stringent laboratory standards.

In summary, the Gilson 402 is a cutting-edge liquid handling solution that combines advanced technology with user-friendly features. Its reliability, precision, and versatility make it an indispensable tool in modern laboratories. Whether used for routine tasks or complex experiments, the Gilson 402 enhances efficiency, promotes accuracy, and ultimately supports groundbreaking scientific research.
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