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Gilson 402 manual Dilutor Dispenser User’s Guide, LT801310/C - 2003 Gilson SAS All rights reserved

Models: 402

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402 Dilutor Dispenser

User’s Guide

LT801310/C - ©2003 Gilson SAS All rights reserved

November 2003

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Gilson 402 manual Dilutor Dispenser User’s Guide, LT801310/C - 2003 Gilson SAS All rights reserved, November

Contents

402 Dilutor Dispenser User’s Guide Page 402 Dilutor Dispenser User’s Guide LT801310/C - 2003 Gilson SAS All rights reservedNovember Page 1 Introduction Table of ContentsSafety 3 InstallationTable of Contents-2 MaintenanceOperation - Standard Use Operation - Advanced Use402 Dual Syringe with Tee Junction Config 7 TroubleshootingAppendices IndexPage Safety-1 SafetySafety Introduction1 Notational Conventions IntroductionNotational Conventions Overview OverviewIntroduction Good Laboratory Practice GLP is facilitated by the following checking protocol for the hydraulic specifica- tions IQ/OQ proceduresIntroduction OverviewUnpacking UnpackingIntroduction Warranty WarrantyIntroduction Customer Service Customer ServiceIntroduction Introduction Description2 Rear Panel Single-syringeModelDescription2 Front PanelFront Panel Dual-syringe ModelDescription Dual-syringe ModelKeypad and Probe Keypad and ProbeKeypad ProbeSyringes Hydraulic ComponentsValve Tee JunctionDescription2 Hydraulic ComponentsThere are seven syringe sizes available for the Installation3 Adapter Kit for Disposable Tips InstallationAssembling the Probe For Standard UseInstallation Assembling the Probe2 Special adapters for the probe 1 D200 tips, pack of Valve Inlet Tubing Connecting the TubingConnecting the Tubing Single-syringe ModelValve Waste Tubing Connecting theDual-syringe Model TubingInstallation Connecting the Tubing1 mL, 5 mL, and 10 mL are available as additional accessories Voltage Selector Installing the FusesElectrical Connections Sector BlockInstallation Connecting the Power CordConnecting the Keypad Connecting the ProbeInstallation SyringesAssembling a Syringe Fitting a SyringeUpgrading to Dual-syringe Model Upgrading to Dual-syringe ModelInstallation Installation Removing the Cover Connecting the Flat CableUpgrading to Dual-syringe Model Upgrading to Dual-syringe Model Inserting the ModuleInstallation 3 Secure the module in place at the top using the two screws providedOperation - Standard Use Standard Parts Fit the Fuses and Connect the PowerEssential Steps Operation - Standard UseOperation - Standard Use Switch-onInitialization Screen Initialization Screen Homing Screen Start-up ScreenOperation - Standard Use Configuration ConfVolumes Configuration Screen Tubing Volume Screen Air Gap Volume ScreenOperation - Standard Use Configuration ScreenSyringes Flow RatesEssential Steps Other ItemsResv Flow Screen Prime Flow Screen Beeper Screen Autorefill Screen Operation - Standard UseIntroduction Configuration Screen Syringe Screen Counter ScreenFit the Syringes Creating a Method EditEntering the Method In-run PromptsMethod Example Start-up Screen Task Selection ScreenTask Selection Screen Rinse Screen Task Edit Screen Save Screen Press ESC, Dispense Screen 7 appearsDispense Screen Dispense Screen Dispense Screen Dispense Screen 4a Dispense Screen 4b Dispense Screen Dispense Screen Dispense ScreenManipulation Screen Initialization Screen Abort Screen Prime the 402 ManPressing End Pressing STOPEssential Steps Running a Method RunRun Screen Run Screen Abort Screen Operation - Standard UseMethod Example In Run Screen In Run Screen 2a In Run Screen 2bOperation - Standard Use Essential StepsEssential Steps In Run Screen 3b In Run Screen 4b In Run Screen 5b In Run Screen 6bAbort Screen Operation - Standard UseEssential Steps Operation - Standard UseOperation - Advanced Use Liquids should be separated by an air gap to minimize contamination Correct Use of aOperation - Advanced Use Correct Use of theCorrect Use of the Left-hand Syringe Aspirating from ReservoirLeft-hand Syringe Dispensing from Reservoir Operation - Advanced UseProbe Aspirating Large Volumes via the ProbeAspirating Small Volumes via the Probe Right-hand Syringe Dispensing toSoftware SoftwareOperation - Advanced Use Configuration Screen Entering the Configuration CONFTubing Volume Screen VolumesSoftware Syringe ScreenChange Size Operation - Advanced UseOperation - Advanced Use Syringe Correction ScreenAir Flow Screen Resv Flow Screen Prime Flow Screen Correction Factorpress Count, the Syringe Counter Screen appears return to the Configuration ScreenCreating a Method EDIT CountersSyringe Correction Screen Save Screen File Menu Screen Saving a MethodFile Manipulations FILE Delete a FileNew File Screen Copy a FileFile Directory Lock or Unlock a FileOperation - Advanced Use Direct Manipulations MANPrime Locked File Screen Action Screen Initialization Screen Abort ScreenSoftware MeasureMeasure Screen Flow Rate Screen Operation - Advanced UseOperation - Advanced Use ProcedureTitrate Measurement ScreenSoftware Tasks - Detailed DescriptionsTitrate Screen Titrate Screen Titrate Screen Titrate Screen Operation - Advanced UseSoftware KeysParameters Operation - Advanced UseParameter Chaining TasksOperation - Advanced Use SoftwareTypical Task Entry DiluteScreens Dilute NormalDilute Serially ENTERNext REAGScreens Operation - Advanced UseSoftware Typical Task EntrySoftware Dilute UnknownTypical Task Entry Operation - Advanced UseRun Procedure Operation - Advanced UseSoftware Dispense Operation - Advanced UseSoftware Operation - Advanced Use SoftwareTypical Task Entry Key to press PipetteOperation - Advanced Use SoftwareSoftware RinseExtra Operation - Advanced UseOperation - Advanced Use LinkWhat it Does ExampleEdit More Link File # 7 ENTER Next File # 8 ENTER ConfigurationPrefabricated Methods Key to PressIn-run Prompts Running a Method RUNOperation - Advanced Use SoftwarePressing STOP When you have finished, or wish to change programs, press STOP, to instantly halt the program. Alterna- tively, you can bring the 402 to a halt by switching off the power, in which case you must ensure that the probe end of transfer-tubing is in a suitable waste container before switching on againOperation - Advanced Use SoftwareAdditional Information In ConfigurationAdditional Information Flow RatesExample Flow Rates - Dual-syringe ModelOperation - Advanced Use Additional InformationAdditional Information Operation - Advanced Use Syringe Correction FunctionUse of Disposable Tips Air ModeLiquid Mode Operation - Advanced UseAdditional Information Cleaning of the valve, Tee, syringe assemblies and the tubings Maintenance6The only maintenance operations necessary for the 402 DD are Cleaning the exterior of the instrumentCleaning the Valve CleaningMaintenance Cleaning the Exterior1 Pull the axle away from the valve main body Cleaning the Tee JunctionMaintenance Cleaning1 Dismount the Syringe assembly from the instrument Cleaning the Syringe AssemblyMaintenance CleaningMaintenance AutoclavingAutoclaving the Tubing AutoclavingMaintenance Changing the Syringe Piston AssemblyChaning the Syringe Piston Assembly Replacing the PistonMaintenance Changing the Piston Syringes and Piston NosesChanging the 100 and 250 µL Piston Noses Changing the Piston Syringes and Piston NosesPiston NosesChanging the Piston MaintenanceChanging the 500 µL Piston Noses Changing the Syringe Piston and Piston NosesMaintenance Maintenance Syringe body Changing the 1 mL Piston NosesPiston Noses Changing the Piston SyringesChanging the Syringe Piston and Piston Noses Changing the 25 mL Piston NosesChanging the 5 mL and 10 mL Piston Noses MaintenanceChanging the Piston Syringes and Piston Noses MaintenanceTroubleshooting7 Mechanical Problems TroubleshootingMechanical Problems Hydraulic Problems Hydraulic ProblemsTroubleshooting Electrical Problems Electrical ProblemsTroubleshooting Appendix A-1 Parts & Accessories Lists Appendix A402 Single Syringe Parts & Accessories Lists402 Single Syringe Appendix AAppendix A 402 Dual Syringe with Tee Junction Configuration402 Dual Syringe with Tee Junction Configuration Parts & Accessories ListsAppendix A AdditionalAccessoriesAdditionalAccessories Parts & Accessories ListsAppendix A Spare PartsSpare Parts Parts & Accessories ListsParts & Accessories Lists Appendix AAppendix A-6 Appendix B-1 Chemical Compatibility Appendix BMaterials in Contact with Liquids Chemical CompatibilityChemical Compatibility Appendix BAppendix C Technical DataHydraulics Technical DataMinimum Volume Maximum VolumeAppendix C Performance SpecificationsProtocol Technical DataAppendix C Junction TubingTechnical Data See OQ Document for procedure and calculationsValve MechanicsMechanics Technical DataConformity to Norms Power RequirementsSize and Weight EnvironmentalAppendix D Error MessagesAppendix D Error MessagesError Messages Error MessagesAppendix D-3 Error MessagesError Messages Appendix DError Messages Appendix DAppendix D-4 Index-1 IndexIndex-2 IndexIndex-3 IndexIndex-4 IndexPage Gilson SAS E-mail sales@gilson.com, service@gilson.com, training@gilson.comWorld Headquarters Gilson, Inc