Gilson 402 manual Autoclaving the Tubing, Maintenance

Maintenance

Autoclaving

Certain parts of the 402 can be autoclaved (20 min. at 0.1 MPa, 121° C). They must be removed from the instrument for autoclaving.

The autoclavable parts are:

•The valve (disassembled) or the Tee-junction.

•The syringe (except the 25 mL syringe).

•The piston assembly.

•PTFE and FEP tubing.

Autoclaving the Tubing

The tubing can be cleaned or autoclaved.

This procedure prevents the end of the tubing from deforming. It is only necessary for the first time you autoclave the tubing.

Precautions to take when autoclaving the tubing:

1)To preserve the flanged end of the tubing, perform the following steps for the first time that you autoclave these components.

2)Insert and tighten the flanged end of the inlet tubing into one end of the PVDF union connector. Tighten the flanged end of the outlet tubing to the other end of the PVDF union connector. Connect the other end of the inlet tubing to the filter.

3)Autoclave the tubing while connected to the union connector.

4)Disconnect the PVDF union connector.

5)Autoclave the tubing a second time to sterilize the interior of the tubing.

Transfer	Tubing		Seal
		Union
tubing	connector	connector	(threaded)

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Autoclaving

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