Gilson 402 manual Hydraulic Components, Valve, Tee Junction, Syringes

Description 2

Hydraulic Components

Valve

The two-position valve, which is fitted to the left- hand side of the 402, has three ports. It switches the syringe port to either the reservoir or to the probe side (see opposite figure).

The valve, which has a 0.5 sec rotation period, is driven by a stepper motor. It is designed to with- stand a million rotations, when used with water. The liquid contact surfaces of the valve, which is autoclavable, are in PEEK and ceramic (121°C, 0.1 MPa, 20 min).

Probe (or Tee

From reservoir junction)

To waste

Syringe

Hydraulic Components

Tee Junction

From Valve To Probe

The Tee-junction, which is fitted to the right-hand side of the dual-syringe model, has three ports: the inlet from the valve, the outlet to the probe, and the syringe port (see opposite figure).

The Tee-junction, which has no internal moving
parts, includes 2 channels, each 1.5 mm in diam-
eter oriented at 120°. The liquid contact surfaces of	Syringe
the Tee-junction, which is autoclavable, are in PEEK.

Syringes

The choice of syringe allows the 402 Dilutor-Dispenser to handle volumes from 1 µL to 25 mL with low values of systematic and random error. The 402 Dilutor-Dispenser is able to transfer liquid as follows:

-from 1 µL to 10 mL from the probe (transfer tubing volume of up to 10 mL),

-up to 25 mL from the reservoir, in one stroke,

-up to 99 mL from the reservoir, in several strokes.

When using the Dual-syringe model, a theoretical dilution ratio of 1/25000 is achieved in one stroke of both sample and diluent syringes. However, you are advised not exceed a ratio of 1/500 for any given dilution, in order to stay within the RSD range (after efficient mixing).

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