Gilson 402 manual Keys, Parameters, Operation - Advanced Use, Software

Operation - Advanced Use 5

Software

Keys

The use of the soft-keys is as follows:

-Prev is used to go to the previous task (or screen within a task),

-Next is used to go to the next task (or screen within a task),

-Add is used to add a task to the Method (after the task displayed),

-Param is used to access the parameters for the current task,

-Ins is used to insert a task (before the task displayed),

-Del is used to remove the task currently displayed.

Parameters

The following list summarizes the parameters used by the tasks; it gives the maximum and minimum values for each parameter. The maximum values are mostly unambiguous. However, in the case of volumes aspirated via the probe, the total volume of fluids (air and liquids) aspirated by a task can never exceed the internal volume of the transfer tubing.

The maximum values listed for volumes aspirated via the probe are guidelines that you should follow when considering parameter values to enter. In some cases you may be able to key in a larger value but this will provoke a self explanatory error message, immediately after entry. Even then, depending on the task, you may key in a series of parameter values, for which the software will then calculate the total volume. If the transfer-tubing volume is exceeded, you will be advised of the required configuration.

For example, you have configured you 402 with the standard 2 mL transfer tubing. In a task, where you can aspirate more than one liquid (e.g. sample and reagents), the total volume of all fluids adds up to 2110 µL. The software issues a warning message (for example, ‘Tubing too small’). You press the OK key to acknowledge the message, after which will you see ‘Parameters Incompatible!’ on the screen. At this point you can either press Quit and reconfigure the 402 or press OK and renter one or more of the volumes entered in the task.

When you configure your 402 for use with dispos- able tips, the ‘Extra volume’ parameter is replaced automatically by ‘Air-push volume’ in the Dilute, Distribute and Pipette tasks, corresponding to the special requirements of this type of configuration; refer to pages 5-33, 5-34 for additional information.

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