402 Dilutor Dispenser User’s Guide
Page
LT801310/C - 2003 Gilson SAS All rights reserved
402 Dilutor Dispenser User’s Guide
November
Page
Safety
Table of Contents
3 Installation
1 Introduction
Operation - Standard Use
Maintenance
Operation - Advanced Use
Table of Contents-2
Appendices
7 Troubleshooting
Index
402 Dual Syringe with Tee Junction Config
Page
Safety-1
Safety
Safety
Introduction1
Introduction
Notational Conventions
Notational Conventions
Overview
Overview
Introduction
Introduction
checking protocol for the hydraulic specifica- tions IQ/OQ procedures
Overview
Good Laboratory Practice GLP is facilitated by the following
Unpacking
Unpacking
Introduction
Warranty
Warranty
Introduction
Customer Service
Customer Service
Introduction
Introduction
Description2
Description2
Single-syringeModel
Front Panel
Rear Panel
Description
Dual-syringe Model
Dual-syringe Model
Front Panel
Keypad
Keypad and Probe
Probe
Keypad and Probe
Valve
Hydraulic Components
Tee Junction
Syringes
Hydraulic Components
Description2
There are seven syringe sizes available for the
Installation3
Assembling the Probe
Installation
For Standard Use
Adapter Kit for Disposable Tips
Assembling the Probe
Installation
2 Special adapters for the probe 1 D200 tips, pack of
Connecting the Tubing
Connecting the Tubing
Single-syringe Model
Valve Inlet Tubing
Dual-syringe Model
Connecting the
Tubing
Valve Waste Tubing
Connecting the Tubing
Installation
1 mL, 5 mL, and 10 mL are available as additional accessories
Electrical Connections
Installing the Fuses
Sector Block
Voltage Selector
Connecting the Keypad
Connecting the Power Cord
Connecting the Probe
Installation
Assembling a Syringe
Syringes
Fitting a Syringe
Installation
Upgrading to Dual-syringe Model
Upgrading to Dual-syringe Model
Installation
Connecting the Flat Cable
Installation Removing the Cover
Upgrading to Dual-syringe Model
Installation
Inserting the Module
3 Secure the module in place at the top using the two screws provided
Upgrading to Dual-syringe Model
Operation - Standard Use
Essential Steps
Fit the Fuses and Connect the Power
Operation - Standard Use
Standard Parts
Initialization Screen Initialization Screen Homing Screen
Switch-on
Start-up Screen
Operation - Standard Use
Volumes
Configuration Conf
Configuration Screen Tubing Volume Screen Air Gap Volume Screen
Operation - Standard Use
Syringes
Configuration Screen
Flow Rates
Operation - Standard Use
Resv Flow Screen Prime Flow Screen Beeper Screen Autorefill Screen
Other Items
Operation - Standard Use
Essential Steps
Fit the Syringes
Configuration Screen Syringe Screen Counter Screen
Creating a Method Edit
Introduction
Method Example
In-run Prompts
Start-up Screen Task Selection Screen
Entering the Method
Dispense Screen Dispense Screen Dispense Screen Dispense Screen 4a
Press ESC, Dispense Screen 7 appears
Dispense Screen 4b Dispense Screen Dispense Screen Dispense Screen
Task Selection Screen Rinse Screen Task Edit Screen Save Screen
Pressing End
Prime the 402 Man
Pressing STOP
Manipulation Screen Initialization Screen Abort Screen
Run Screen Run Screen Abort Screen
Running a Method Run
Operation - Standard Use
Essential Steps
Operation - Standard Use
In Run Screen In Run Screen 2a In Run Screen 2b
Essential Steps
Method Example
Abort Screen
In Run Screen 3b In Run Screen 4b In Run Screen 5b In Run Screen 6b
Operation - Standard Use
Essential Steps
Essential Steps
Operation - Standard Use
Operation - Advanced Use
Operation - Advanced Use
Correct Use of a
Correct Use of the
Liquids should be separated by an air gap to minimize contamination
Left-hand Syringe Dispensing from Reservoir
Left-hand Syringe Aspirating from Reservoir
Operation - Advanced Use
Correct Use of the
Aspirating Small Volumes via the Probe
Aspirating Large Volumes via the Probe
Right-hand Syringe Dispensing to
Probe
Software
Software
Operation - Advanced Use
Tubing Volume Screen
Entering the Configuration CONF
Volumes
Configuration Screen
Change Size
Syringe Screen
Operation - Advanced Use
Software
Air Flow Screen Resv Flow Screen Prime Flow Screen
Syringe Correction Screen
Correction Factor
Operation - Advanced Use
Creating a Method EDIT
return to the Configuration Screen
Counters
press Count, the Syringe Counter Screen appears
File Manipulations FILE
Saving a Method
Delete a File
Syringe Correction Screen Save Screen File Menu Screen
File Directory
Copy a File
Lock or Unlock a File
New File Screen
Prime
Direct Manipulations MAN
Locked File Screen Action Screen Initialization Screen Abort Screen
Operation - Advanced Use
Measure Screen Flow Rate Screen
Measure
Operation - Advanced Use
Software
Titrate
Procedure
Measurement Screen
Operation - Advanced Use
Titrate Screen Titrate Screen Titrate Screen Titrate Screen
Tasks - Detailed Descriptions
Operation - Advanced Use
Software
Parameters
Keys
Operation - Advanced Use
Software
Operation - Advanced Use
Chaining Tasks
Software
Parameter
Screens
Dilute
Dilute Normal
Typical Task Entry
Next
ENTER
REAG
Dilute Serially
Software
Operation - Advanced Use
Typical Task Entry
Screens
Typical Task Entry
Dilute Unknown
Operation - Advanced Use
Software
Operation - Advanced Use
Run Procedure
Software
Operation - Advanced Use
Dispense
Software
Software
Operation - Advanced Use
Typical Task Entry
Operation - Advanced Use
Pipette
Software
Key to press
Extra
Rinse
Operation - Advanced Use
Software
What it Does
Link
Example
Operation - Advanced Use
Prefabricated Methods
Configuration
Key to Press
Edit More Link File # 7 ENTER Next File # 8 ENTER
Operation - Advanced Use
Running a Method RUN
Software
In-run Prompts
Operation - Advanced Use
When you have finished, or wish to change programs, press STOP, to instantly halt the program. Alterna- tively, you can bring the 402 to a halt by switching off the power, in which case you must ensure that the probe end of transfer-tubing is in a suitable waste container before switching on again
Software
Pressing STOP
Additional Information
In Configuration
Flow Rates
Additional Information
Operation - Advanced Use
Flow Rates - Dual-syringe Model
Additional Information
Example
Use of Disposable Tips
Operation - Advanced Use Syringe Correction Function
Air Mode
Additional Information
Operation - Advanced Use
Liquid Mode
Additional Information
The only maintenance operations necessary for the 402 DD are
Maintenance6
Cleaning the exterior of the instrument
Cleaning of the valve, Tee, syringe assemblies and the tubings
Maintenance
Cleaning
Cleaning the Exterior
Cleaning the Valve
Maintenance
Cleaning the Tee Junction
Cleaning
1 Pull the axle away from the valve main body
Maintenance
Cleaning the Syringe Assembly
Cleaning
1 Dismount the Syringe assembly from the instrument
Autoclaving the Tubing
Autoclaving
Autoclaving
Maintenance
Chaning the Syringe Piston Assembly
Changing the Syringe Piston Assembly
Replacing the Piston
Maintenance
Changing the 100 and 250 µL Piston Noses
Changing the Piston Syringes and Piston Noses
Changing the Piston Syringes and Piston Noses
Maintenance
Changing the Piston
Noses
Maintenance
Piston
Changing the Syringe Piston and Piston Noses
Changing the 500 µL Piston Noses
Maintenance
Piston Noses
Syringe body Changing the 1 mL Piston Noses
Changing the Piston Syringes
Maintenance
Changing the 5 mL and 10 mL Piston Noses
Changing the 25 mL Piston Noses
Maintenance
Changing the Syringe Piston and Piston Noses
Changing the Piston Syringes and Piston Noses
Maintenance
Troubleshooting7
Troubleshooting
Mechanical Problems
Mechanical Problems
Hydraulic Problems
Hydraulic Problems
Troubleshooting
Electrical Problems
Electrical Problems
Troubleshooting
Appendix A-1
Parts & Accessories Lists Appendix A
402 Single Syringe
Parts & Accessories Lists
Appendix A
402 Single Syringe
402 Dual Syringe with Tee Junction Configuration
402 Dual Syringe with Tee Junction Configuration
Parts & Accessories Lists
Appendix A
AdditionalAccessories
AdditionalAccessories
Parts & Accessories Lists
Appendix A
Spare Parts
Spare Parts
Parts & Accessories Lists
Appendix A
Appendix A
Parts & Accessories Lists
Appendix A-6
Appendix B-1
Chemical Compatibility Appendix B
Chemical Compatibility
Chemical Compatibility
Appendix B
Materials in Contact with Liquids
Appendix C
Technical Data
Minimum Volume
Technical Data
Maximum Volume
Hydraulics
Protocol
Performance Specifications
Technical Data
Appendix C
Technical Data
Junction Tubing
See OQ Document for procedure and calculations
Appendix C
Mechanics
Mechanics
Technical Data
Valve
Size and Weight
Power Requirements
Environmental
Conformity to Norms
Appendix D
Error Messages
Error Messages
Error Messages
Error Messages
Appendix D
Error Messages
Error Messages
Appendix D
Appendix D-3
Appendix D
Error Messages
Appendix D-4
Index-1
Index
Index-2
Index
Index-3
Index
Index-4
Index
Page
World Headquarters
E-mail sales@gilson.com, service@gilson.com, training@gilson.com
Gilson, Inc
Gilson SAS