Gilson 402 manual Dilute, Screens, Key to press

Operation - Advanced Use 5

Software

If you specify a volume of sample greater than that of the right- hand syringe, the left-hand will be used to aspirate the sample.

14

diluent result

2

air gap

3

sample

Screens:

Dilute

Three types of dilution task are available ‘Normal’, ‘Unknown’, and ‘Serial’. Three modes are available for ‘Normal’ and ‘Serial’: Total Volume, Diluent Volume, and Dilution Ratio. The first action is to select the type of dilution task that you are going to use, by pressing the appropriate soft-keys.

Dilute Normal

You use Dilute Normal to dilute samples, with diluent from the reservoir or via the probe, and add up to 12 reagents. There are 3 modes of entering the parameters, in each mode you supply Sample Volume (Vs) plus one of the following variables: Dilution Ratio (Rd), Diluent Volume (Vd), and Total Volume (Vt). The software calculates the remaining variables, taking into account the volumes of the added reagents (Vr).

Vt = Vs x Rd, Vd = Vt - Vs - Vr

For example, after selecting Dilution Ratio Mode, you enter Sample Volume = 10 and Dilution Ratio = 3, Reagent Volume (total) = 9; the software calculates the Total Volume as 30 and the required Diluent Volume as 11. In any mode, pressing the Data soft- key gives the calculated values for the other two parameters.

1.Aspirate the required volume of diluent (in opposite figure, from the reservoir).

2.Aspirate an air gap (specified in configuration).

3.Possibly, Aspirate reagent(s) then the sample (with an air gap between each liquid).

4.Dispense all into recipient vessel.

Typical Task Entry

Key to press:

Key in the File # (e.g. 48), Press ENTER

Press Edit

Press Dil Press Normal

Press Chge to select source, press ENTER, then Next.

Press Chge to select the mode, press ENTER,

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