Gilson 402 manual Flow Rates Dual-syringe Model

Operation - Advanced Use 5

Additional Information

Maximum Dispense Flow Rate

(No Air Gaps)

Tubing Type			Syringe Volume (µL)
	100	250	500	1000	5000	10000	25000
1 mL	4	10	20	40	120	120	120
2 mL	4	10	20	40	120	120	120
5 mL	4	10	20	40	120	120	120
10 mL	4	10	20	40	120	120	120
Micro Tips (liquid)	4	10	20	40	120	120	120
Micro Tips (air)	4	10	20	40	120	120	120
Large Tips (air)	4	10	20	40	120	120	120

Recommended Flow Rate with an Air Gap (10 µL)

Syringe size			Flow rate
			(mL/min)
100 µL			4
250 µL			10
500 µL			20
1 mL			30
5 mL		Asp: 30 - Disp: 50
10	mL	Asp: 30 - Disp: 50
25	mL	Asp: 30 - Disp: 50

Flow Rates - Dual-syringe Model

Flow rates are the values entered in Flow menus (Asp Flow or Disp Flow).

•It is not possible to enter a flow rate greater than the maximum without entering an air gap for the left-hand syringe.

•If a flow rate (aspirate or dispense) is greater than the maximum for the right-hand syringe, the left- hand syringe aspirates and dispenses at the selected flow rate. The right-hand syringe aspirates and dispenses as its maximum flow rate.

•If a flow rate is equal to or smaller than the maximum for the right-hand syringe, both syringes aspirate and dispense at the selected flow rate.

Example

When using the 2 mL tapered transfer-tubing,

a5 mL syringe at the left-hand side and a 500 µL syringe at the right-hand side, the default aspirate flow rates are respectively 100 mL/min and

20 mL/min. The dispense flow rate, accessible by pressing Deflt soft-key, is 120. The implicit flow rate of the small syringe is 20 mL/min.

If the value of the flow rate is between 120 and 20, the flow rate of the left-hand syringe takes this value and the flow rate of the right-hand syringe is 20. To reduce to the flow rate of the right-hand syringe, it is necessary to enter values below 20 mL/min.

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