Gilson 402 manual In Run Screen In Run Screen 2a In Run Screen 2b, Operation - Standard Use

Operation - Standard Use 4

Essential Steps

In Run Screen (1)

In Run Screen (2a)

In Run Screen (2b)

Ensure that the probe-end of the transfer-tubing is in a suitable waste container, then press OK. ‘Homing ...’ appears on the screen until the syringe(s) have returned to their home positions, during which time any liquid contained in the transfer-tubing is expelled from the probe-end. After this the Action Screen reappears.

Press ESC to return to the start-up screen for the current file.

Whenever you see ‘Homing’ on the screen, the syringe piston(s) are returned to their uppermost position(s). As a result, some (or all) of the liquid contained in the transfer-tubing will be expelled from the probe-end. You must ensure that the probe end of transfer-tubing is already in a suitable waste container.

Method Example

This section describes how to run Method 33 (created as above).

(i)Press Run, you see In Run Screen 1.

M33/T 1: DISPENS/1 means Method 33 Task 1, Dispense Task, first cycle.

The cursor moves from side-to-side below the parameter value field, indicating that you must enter a value (or validate the current value, in this example 6 mL/min.). Key in the required flow rate (for example, 12 mL/min.) and press ENTER. [Similar screens would appear for any other parameters that you had entered as a question mark (?).] The command light flashes and you see alternately In Run Screens 2a and 2b (about every half a second).

In the Dispense task, you aspirate an air gap, then liquid. After aspirating, you dispense the liquid in several fractions, finally the ‘Extra volume’ is sent to waste.

1					Extra Volume
air gap				7
	6			to waste
			fraction 4
	5
2		fraction 3
liquid	4
	fraction 2
	3
	fraction1

4-12