Operation - Advanced Use

5

 

 

 

Software

Measurement Screen

1High precision is obtained by performing this action at a low flow rate. And, because the piston accelerates after pressing the command button, you are advised to start-and-stop dispensing by repeatedly pushing the command button with an increasing frequency as the liquid nears the tip.

After completely aspirating the liquid being measured, you see (for example) the Measurement Screen.

Procedure

1)Aspirate all the liquid contained in the source vial, plus an air cushion, to ensure that you have aspirated all the liquid. You should press the command button to stop aspirating when you can see the air/liquid interface in the transfer tubing.

2)Press the Asp key to reverse the direction of flow; (Asp is replaced by Disp).

3)With the tip of the tubing in the vial, press the command button to start dispensing the air cushion.

4)While carefully watching the air/liquid inter- face, press the command button to stop dispens- ing at the instant the liquid arrives at the tip of the tubing1.

5)Read the volume shown on the screen (496.7 µL in the example).

6)With the tip of the tubing in the vial, press the command button to dispense the liquid back into the source vial.

7)Before repeating this procedure with other vials, remember to reverse the direction of flow by pressing the Disp key again (Disp is replaced by Asp).

Conclude the current measurement by pressing STOP or End (see Prime for details).

Titrate

The use of this direct manipulation is the same as classical titration (using a burette) where the idea is to run a quantity of reagent into a sample that includes a pH indicator, up to the point where the color changes. With 402, you may also use titrate to fill plates, vials, or bottles.

To titrate, you aspirate a known volume of reagent, dispense part of the volume required into the sample, agitate the sample, dispense a small amount, agitate again, dispense a smaller amount, agitate again, etc., as you move in finer and finer steps towards the point where you have added the precise volume required to change the color.

5-14

Page 63 Page 65
Page 64
Image 64
Gilson 402 manual Procedure, Titrate, Measurement Screen
Page 63 Page 65

402 specifications

The Gilson 402 is a highly regarded laboratory device known for its precision and reliability in liquid handling applications. Primarily utilized in research and clinical labs, this automated pipetting system significantly enhances productivity by reducing the time taken for repetitive tasks while ensuring maximum accuracy.

One of the standout features of the Gilson 402 is its intuitive user interface. The touchscreen display allows operators to easily navigate through various functions and settings, enabling quick setup and adjustments. Its intuitive design minimizes the learning curve for new users, making it accessible even for those with limited technical expertise.

The Gilson 402 employs advanced pipetting technologies, which provide exceptional reproducibility and reliability. The system is equipped with proprietary Pipette G (as used in Gilson's PIPETMAN series), which ensures precise volume delivery. This is crucial in applications such as molecular biology, where even minor discrepancies in liquid volumes can lead to significant variations in experimental outcomes.

Another key characteristic of the Gilson 402 is its versatility. The device accommodates a wide range of pipette tips, allowing users to work with varying volumes and types of samples. Whether handling delicate biological fluids or viscous solutions, the Gilson 402 adapts to different protocols seamlessly.

The automation capabilities extend to a customizable programming feature that permits users to create complex pipetting protocols with ease. Multiple-step pipetting tasks can be completed effortlessly, reducing the likelihood of errors caused by manual handling.

Equipped with high-precision motors and sensors, the Gilson 402 also features built-in calibration and quality control functions. This guarantees that the system remains accurate over time and can easily be adjusted to maintain compliance with stringent laboratory standards.

In summary, the Gilson 402 is a cutting-edge liquid handling solution that combines advanced technology with user-friendly features. Its reliability, precision, and versatility make it an indispensable tool in modern laboratories. Whether used for routine tasks or complex experiments, the Gilson 402 enhances efficiency, promotes accuracy, and ultimately supports groundbreaking scientific research.
Top Page Image Contents