Gilson 402 Correct Use of a, Operation - Advanced Use, Correct Use of the, Single-syringe Model

Operation - Advanced Use 5

Correct Use of the 402

(a) Aspirating from Reservoir

From Reservoir

Correct Use of a 402

Single-syringe Model

•Samples and reagents are aspirated from the probe side.

•Liquid aspirated from the probe side may not enter the syringes. Thus, the tubing that connects the syringe to the probe, called transfer tubing, has to be carefully chosen to accommodate the volumes of samples and reagents defined in the Method.

•Liquids should be separated by an air gap to minimize contamination.

•If a drop of liquid is present at the end of the tubing after dispensing, you must include this drop in the result vial.

•Syringe functionality is shown below.

(b) Aspirating from Probe	(c) Dispensing
From Probe	To Probe

Piston descends	Piston descends
to aspirate	to aspirate

Dual-syringe Model

Piston ascends to aspirate

•Samples and reagents are aspirated from the probe side.

•Liquid aspirated from the probe side may not enter the syringes. Thus, the tubing that connects the syringe to the probe, called transfer tubing, has to be carefully chosen to accommodate the volumes of samples and reagents defined in the Method.

•Liquids should be separated by an air gap to minimize contamination.

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