Operation - Advanced Use

Press Abort, when you want to stop priming; Initialization Screen 2 reappears. Ensure that the probe-end of the transfer-tubing is in a suitable waste container, then press OK. ‘Homing ...’ appears on the screen until the syringe(s) have returned to their uppermost (home) positions, during which time any liquid contained in the transfer-tubing is expelled from the probe-end. After this the Action Screen reappears.

Press ESC to return to the start-up screen for the current file.

Whenever you see ‘Homing’ on the screen, the syringe piston(s) are returned to their uppermost position(s). As a result, some of the liquid con- tained in the transfer-tubing will be expelled from the probe-end. You must ensure that the probe end of transfer-tubing is already in a suitable waste container.

5

Software

Measure

You can measure a volume (e.g. supernatant after centrifugation).

•Press Measure, the Measure Screen appears.

For a Dual-syringe 402, press the Chge soft-key to select LEFT or RIGHT. You are advised to select the left-hand syringe if the volume to be measured seems to be significantly larger than the capacity of the right-hand syringe.

The maximum volume that you can measure is limited to the volume of the aspirating syringe or the transfer tubing size, whichever is the smaller.

•Press ENTER, the Flow Rate Screen appears.

Key in the flow rate at which you want the volume measurement to take place, and then press ENTER. The default flow rate is half the maximum possible for the combination of syringe and transfer tubing in use (refer to page 5-31).

Press Run, then follow the instructions given on the screen; you press the command button on the probe to aspirate, when prompted to do so by the com- mand light on the probe:

-a steady light means you are to aspirate (or dispense) liquid,

-a flashing light means you are to aspirate air via the probe.

Measure Screen

Flow Rate Screen

5-13

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Gilson 402 manual Measure Screen Flow Rate Screen
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402 specifications

The Gilson 402 is a highly regarded laboratory device known for its precision and reliability in liquid handling applications. Primarily utilized in research and clinical labs, this automated pipetting system significantly enhances productivity by reducing the time taken for repetitive tasks while ensuring maximum accuracy.

One of the standout features of the Gilson 402 is its intuitive user interface. The touchscreen display allows operators to easily navigate through various functions and settings, enabling quick setup and adjustments. Its intuitive design minimizes the learning curve for new users, making it accessible even for those with limited technical expertise.

The Gilson 402 employs advanced pipetting technologies, which provide exceptional reproducibility and reliability. The system is equipped with proprietary Pipette G (as used in Gilson's PIPETMAN series), which ensures precise volume delivery. This is crucial in applications such as molecular biology, where even minor discrepancies in liquid volumes can lead to significant variations in experimental outcomes.

Another key characteristic of the Gilson 402 is its versatility. The device accommodates a wide range of pipette tips, allowing users to work with varying volumes and types of samples. Whether handling delicate biological fluids or viscous solutions, the Gilson 402 adapts to different protocols seamlessly.

The automation capabilities extend to a customizable programming feature that permits users to create complex pipetting protocols with ease. Multiple-step pipetting tasks can be completed effortlessly, reducing the likelihood of errors caused by manual handling.

Equipped with high-precision motors and sensors, the Gilson 402 also features built-in calibration and quality control functions. This guarantees that the system remains accurate over time and can easily be adjusted to maintain compliance with stringent laboratory standards.

In summary, the Gilson 402 is a cutting-edge liquid handling solution that combines advanced technology with user-friendly features. Its reliability, precision, and versatility make it an indispensable tool in modern laboratories. Whether used for routine tasks or complex experiments, the Gilson 402 enhances efficiency, promotes accuracy, and ultimately supports groundbreaking scientific research.
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