Gilson 402 manual Measure Screen Flow Rate Screen, Operation - Advanced Use, Software

Operation - Advanced Use

Press Abort, when you want to stop priming; Initialization Screen 2 reappears. Ensure that the probe-end of the transfer-tubing is in a suitable waste container, then press OK. ‘Homing ...’ appears on the screen until the syringe(s) have returned to their uppermost (home) positions, during which time any liquid contained in the transfer-tubing is expelled from the probe-end. After this the Action Screen reappears.

Press ESC to return to the start-up screen for the current file.

Whenever you see ‘Homing’ on the screen, the syringe piston(s) are returned to their uppermost position(s). As a result, some of the liquid con- tained in the transfer-tubing will be expelled from the probe-end. You must ensure that the probe end of transfer-tubing is already in a suitable waste container.

5

Software

Measure

You can measure a volume (e.g. supernatant after centrifugation).

•Press Measure, the Measure Screen appears.

For a Dual-syringe 402, press the Chge soft-key to select LEFT or RIGHT. You are advised to select the left-hand syringe if the volume to be measured seems to be significantly larger than the capacity of the right-hand syringe.

The maximum volume that you can measure is limited to the volume of the aspirating syringe or the transfer tubing size, whichever is the smaller.

•Press ENTER, the Flow Rate Screen appears.

Key in the flow rate at which you want the volume measurement to take place, and then press ENTER. The default flow rate is half the maximum possible for the combination of syringe and transfer tubing in use (refer to page 5-31).

Press Run, then follow the instructions given on the screen; you press the command button on the probe to aspirate, when prompted to do so by the com- mand light on the probe:

-a steady light means you are to aspirate (or dispense) liquid,

-a flashing light means you are to aspirate air via the probe.

Measure Screen

Flow Rate Screen

5-13