Gilson 402 manual Dispense, Operation - Advanced Use, Software

Operation - Advanced Use 5

If for any reason you want to repeat the measure- ment (e.g. you need to select the LEFT syringe instead of the RIGHT), put tip of the transfer- tubing back into the original container, then press Redo to discharge the liquid.

When you are ready to dilute the previ- ously measured sample, press End. The command light is steady and you see alternately the next pair of screens.

Press the command button, diluent is drawn from the reservoir; the command light is steady and you see alternately the next pair of screens.

Put the tip of the transfer-tubing back into a suitable recipient vial then press the command button to dispense the diluted sample. After which you are returned to the following screen for the next cycle. If you want to quit at this point you must press the STOP key.

Dispense

Software

This task is used to dispense liquid from the reservoir or from the probe, in equal or un- equal fractions, into one or more vessels. You may adjust the final volumes in the recipient vessels to a predetermined final volume, with liquid via the reservoir or the probe. If the source is the reservoir, you can dispense a volume greater than the capacity of the left- hand (or only) syringe, which will complete this operation with several strokes of the piston.

Adjust 1

Adjust 2

Fraction 1

Adjust 3

Fraction 2

Final

Volume

Fraction 3

When you use this task to aspirate and dispense via the probe, you may also aspirate an ‘Extra volume’. The ‘Extra volume’ is aspirated to ensure that the accuracy of the first volume of liquid is maintained (when it is dispensed) and that the first fraction is not contaminated by liquid coming from the reservoir. The ‘Extra volume’, which is aspirated with the first

1

air gap

6

fraction 4

	5
2		fraction 3
liquid	4
	fraction 2
	3
	fraction1

7

to waste

Extra Volume

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