Gilson 402 manual In-run Prompts, Method Example, Start-up Screen Task Selection Screen

Operation - Standard Use 4

Essential Steps

Start-up Screen

Task Selection Screen

In-run Prompts

You can defer some parameters (volumes and flow rates) by entering a question mark (?) in place of the value. You enter the actual value after pressing the RUN key. This feature is particularly useful when optimizing parameters. In the following example, the aspirate flow rate (Asp Flow) is submitted as an unknown. Then, each time the Method is run, you can submit a different value (6, 12, 18). After which you evaluate the results and select the most appro- priate value for use in the Method.

Method Example

Dispense equal volumes of various samples, rinse the probe after distribution.

Common (Fraction) Volume = 20 µL, Number of fractions = 3.

Aspirate flow rate from 6 to 18 mL/minute, dispense flow rate = 6 mL/minute.

In this example ‘Aspirate flow rate’ will be submit- ted as an in-run prompt.

Extra volume = 5 µL.

You aspirate an extra volume of sample, to avoid diluting the last fraction with solvent. This extra volume is sent to the Waste, after distributing the last fraction.

Rinse with 500 µL of solvent.

When using the software, remember that the ‘HELP’ key is available with most screens.

Entering the Method

Beginning at the Start-up Screen, key in the file number for your Method, then press ENTER.

Then press Edit; the Task Selection Screen appears.

You can now access the screens for the required task and enter the task related parameters. The following screens show how to enter the informa- tion (described above) for each task.

In general you must press the Chge soft-key to select an option, then press ENTER to confirm the option selected. Press the Next soft-key to cycle forward through the screens, press Prev to cycle backwards, or ESC when you reach the extremity of a software branch.

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