Gilson 402 manual Left-hand Syringe Aspirating from Reservoir

Operation - Advanced Use 5

•If a drop of liquid is present at the end of the tubing after dispensing, you must include this drop in the result vial.

•The larger capacity syringe must always be mounted on the left-hand side.

•Only the left-hand syringe aspirates the liquid from the reservoir (diluent and rinsing liquid).

•If the capacity of the right-hand syringe is superior to the total volume aspirated (volumes of samples, reagents, air gaps), the right-hand syringe aspirates and delivers liquids from the probe.

•If the capacity of the right-hand syringe is inferior to the total volume to be aspirated (including air gaps), the left-hand syringe will be used to aspirate all liquids.

•Syringe functionality is shown below, steps (a) to (e).

(a)Left-hand Syringe Aspirating from Reservoir

Correct Use of the 402

Left-hand Syringe Aspirating (from Reservoir)

From Reservoir

1.The valve switches to connect the left-hand syringe to the reservoir.

2.The piston of the right-hand syringe remains in the top position.

3.The left-hand piston descends to aspirate the specified volume of liquid from the reservoir.

Left-hand Syringe Dispensing (from Reservoir)

1.The valve switches to connect the left-hand syringe to the Tee-junction and probe.

2.The piston of the right-hand syringe remains in the top position to prevent liquid from entering the syringe.

3.The left-hand piston ascends to dispense the specified volume of liquid from the reservoir.

Left piston			Right piston
descends			remains at
to aspirate			the top
		(b) Left-hand Syringe
			Dispensing
	To Tee		To Probe

Left piston	Right piston
ascends	remains at
to dispense	the top

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