Leica DMI3000B, DMI6000B, DMI4000B manual Adjusting the field diaphragm

7. Start-up

7.6.2Incident Light - Fluorescence

(Leica DMI4000 B and Leica DMI6000 B)

Suitable aperture and field diaphragm values have been preset for each objective. The inci- dent light module has also been centered at the factory.

However, it may be necessary to readjust the in- cident light module in some cases after trans- porting and setting up the stand. Therefore, check the field diaphragm centering.

The following procedure is provided for the inci- dent light-bright field illumination.

¥Select an objective with moderate magnifica- tion (10xÐ20x).

¥Activate the incident-light axis with the TL/IL button (84.1).

¥Press the IL-BF / Fluo button to activate the bright field contrast method (one of the vari- able function buttons on the stand).

¥Place a specimen on the stage.

¥Focus the specimen using the SmartMove or the focus wheels.

Adjusting the field diaphragm

¥Close the field diaphragm with the FD button (84.4) or manually until the edge of the dia- phragm (round or rectangular) appears in the field of view.

¥If the limits of the field diaphragm are not in the center of the field of view, move the posi- tion of the field diaphragm to the center with the two centering screws (92.1) on the right side of the stand.

¥Use the function buttons FD (84.4) to open the field diaphragm to the point that they just dis- appear from the field of view.

¥We recommend the use of a rectangular field diaphragm when using a digital camera. Match the size of the diaphragm to the chip size of the camera.

Fig. 92 Adjusting the field diaphragm (incident light-fluorescence)

1 Adjusting screws for moving the field diaphragm

¥Adjust the light intensity with the INT buttons (84.3).

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