Leica DMI3000B manual Changing the fluorescence filter cube, Leica Application Suite LAS Software

8. Operation

8.3 Fluorescence (Leica DMI4000 and DMI6000 B)

¥Use the TL/IL function button to switch to fluo- rescence FLUO.

¥Place a specimen on the stage and select a suitable objective.

¥The current fluorescence filter cube will be displayed on the LeicaScreen.

¥You may protect your specimen from fading by closing the incident light shutter.

To do so, press the SHUTTER button (87.3) on the front panel.

The following pictogram will appear on the LeicaScreen:

￿Changing the fluorescence filter cube

￿Fixed function buttons on the front panel: CUBE 1 to CUBE 6 or Cube CCW

￿Variable function buttons on the front panel and SmartMove: CUBE CW or CUBE

CCW

￿Leica Application Suite (LAS) Software

¥Focus the image with the knob on the SmartMove or the focusing wheel and adjust the intensity with the INT function buttons.

Options

¥The intensity of the fluorescence can be in- creased by using the booster lens (Fig. 112) on the left rear side of the stand (Fig. 113).

If bright fluorescence is required in the center of the field of view, slide the booster lens into the receptacle with the marking

¥ 1.4x

facing the user. If a homogeneous distribution over the entire field of view is required, turn the booster lens 180° so that the marking

0.7x

is facing forward.

¥For multiple fluorescence, we recommend us- ing the Excitation Manager and/or the ultrafast internal filter wheel. Excitation wave- lengths can thus be changed in milliseconds. They are controlled by the function buttons.

Fig. 112 Booster lens Fig. 113 Booster lens in stand

1

90