Leica DMI3000B, DMI6000B manual Manual alternative, DIC disk with knurled wheel for fine adjustment

8. Operation

8.2.5 Differential Interference Contrast (TL)

Leica DMI3000 B:

¥Select an objective.

¥At the condenser, select the appropriate Wollaston prism condenser.

¥At the objective turret, select the appropriate Wollaston prism objective.

¥Move the polarizer on the condenser into the beam path.

¥Insert the analyzer into the right side of the stand until it clicks into position.

¥Insert a specimen.

¥Set the brightness at the light potentiometer

¥Focus the image with the focus wheels.

¥Use the knurled wheel below the objective turret for fine adjustment (Fig. 111).

Leica DMI4000 B and Leica DMI6000 B:

¥Use the TL/IL function button to switch to transmitted light (TL).

¥Select the DIC contrast method

by pressing the variable button DIC. Alternatively: press the variable button

CHANGE TL .

(For details on button assignments, please see the identification sheet.)

DIC will appear on the LeicaScreen.

¥The polarizer in the condenser and the suit- able condenser prism are automatically posi- tioned in the beam path. The corresponding objective prism and the analyzer cube are also positioned automatically.

¥Place a DIC specimen on the stage.

¥Rotate an appropriate objective into place.

¥Focus the image with the knob on the SmartMove or the focusing wheel and adjust the intensity with the INT function buttons.

¥Use the knurled wheel below the objective turret for fine adjustment (Fig. 111).

Manual alternative:

¥Move the polarizer on the condenser into the beam path manually.

¥Insert the analyzer manually into the right side of the stand until it clicks into position (Fig. 110). Adjust the objective and condenser prisms manually until a valid combination appears on the display.

¥Use the knurled wheel below the objective turret for fine adjustment (Fig. 111).

Fig. 111 DIC disk with knurled wheel for fine adjustment

87