7. Start-up

7.8Checking modulation contrast slit diaphragms

If your microscope is prepared for integrated modulation contrast, its condenser will be equipped with slit diaphragms suitable for the objectives.

The slit diaphragms have been centered at the factory.

Their proper location should be checked, how- ever.

Note:

Each objective has its own slit diaphragm as- signed to it in the condenser disk. The test must therefore be performed for each objective.

Open the cover at the top right side of the con- denser. The various

numbered openings for the inserts are now vis- ible. Ensure that all of the slit diaphragms are firmly seated and that none of the retaining screws are loose. If a part has loosened, please see Chapter 6.5 Installation of Condensers.

7.9 Setting the Motorized Polarizer

Remove your specimen from the stage.

Leica DMI3000 B:

¥Set the condenser to the bright field position.

¥Insert the analyzer into the analyzer slot on right side of the stand.

¥Activate the polarizer.

¥Turn the polarizer until you have the optimal dark position.

Leica DMI4000 B and Leica DMI6000 B:

For manual condensers, proceed as described above for the DMI3000 B.

¥Select the POL method (one of the variable function buttons on the stand). If the analyzer is present on the Fluo turret as an analyzer block, it will move into position automatically. A manual analyzer must be positioned by hand.

In the case of motorized condensers with mo- torized polarizers, the polarizer will move into position automatically.

¥Insert the centering key in the opening pro- vided on the condenser (Fig. 96).

¥Set up optimal darkening. (The analyzer must be in place.)

¥Remove the centering keys.

Replace your specimen on the stage.

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Leica DMI6000B, DMI3000B, DMI4000B Start-up Checking modulation contrast slit diaphragms, Setting the Motorized Polarizer