8. Operation

8.2.6 Integrated Phase Contrast (TL)

Leica DMI3000 B:

¥Select a bright field objective with eyepoint A or C.

¥Select the appropriate light ring at the con- denser (see table).

¥Open the aperture of the condenser com- pletely.

¥Remove all other optical components such as analyzers, polarizers or IC prisms from the beam path.

¥Slide the phase contrast front module to the correct eyepoint, A or C.

¥Insert a phase contrast specimen.

¥Set the brightness at the light potentiometer

¥Focus the image with the focus wheels.

Leica DMI4000 B and Leica DMI6000 B:

¥Use the TL/IL function button to switch to transmitted light (TL).

¥Select the IPC contrast method (integrated phase contrast). by pressing the variable but- ton IPH. Alternatively: press the variable but- ton CHANGE TL . (For details on button as- signments, please see the identification sheet.)

PH will appear on the LeicaScreen. Motorized condensers will now switch to the correct light ring. Coded condensers must be switched manually.

¥Insert a transmitted light specimen.

¥Select a suitable objective (eyepoint A or C).

¥Slide the phase contrast front module to the correct eyepoint, A or C.

¥Focus the image with the knob on the SmartMove or the focusing wheel and adjust the intensity with the INT function buttons.

 

 

 

 

 

Note:

 

 

 

When selecting the phase contrast method, the

 

 

 

aperture diaphragm is opened fully and can not

 

 

 

be adjusted.

 

 

 

 

 

 

 

IP0

for 5x,

e.g. NPlan 5x

11506087

 

objective with eyepoint A

 

 

 

 

 

 

IP1

for 10x,

e.g. NPlan 10 x

11506084

 

objective with eyepoint A and

 

for 20x,

e.g. NPlan L 20 x

11506200

 

objective with eyepoint C

 

 

 

 

 

 

IP2

for 40x,

e.g. HCX PL FL L 40 x

11506201

 

objective with eyepoint C

 

 

 

 

 

 

IP3

for 63x,

e.g. PL FL 63x/0.70

11506216

 

objective with eyepoint C

 

 

 

 

 

 

88

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Leica DMI6000B, DMI3000B, DMI4000B manual Operation Integrated Phase Contrast TL
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