8. Operation

8.2.4 Polarization (TL)

Leica DMI3000 B:

¥Select an objective.

¥Set the condenser to the bright field position.

Remove all IC prisms from the light path.

¥Move the polarizer on the condenser into the beam path.

¥Insert the analyzer into the right side of the stand until it clicks into position.

Manual method:

¥Move the polarizer on the condenser into the beam path.

¥Insert the analyzer into the right side of the stand until it clicks into position (Fig. 110).

¥Bring the polarizer and analyzer into cross po- sition until they reach maximum darkness.

¥Place a specimen on the stage and select a suitable objective.

¥Bring the polarizer and analyzer into cross po- sition until they reach maximum darkness.

¥Insert a specimen.

¥Set the brightness at the light potentiometer

¥Focus the image with the focus wheels.

Leica DMI4000 B and Leica DMI6000 B:

¥Use the TL/IL function button to switch to transmitted light (TL).

¥Select the POL (polarization) contrast method by pressing the variable button POL. Alternatively: press the variable button

CHANGE TL .

(For details on button assignments, please see the identification sheet.)

POL will appear on the LeicaScreen.

Motorized method:

¥If the microscope is equipped with the rel- evant components, the polarizer will be acti- vated automatically in the condenser when the POL contrast method is selected. The analyzer cube is also automatically positioned in the beam path.

Combined methods:

¥The Leica DMI4000 B and Leica DMI6000B mi- croscope permit purely mechanical and mo- torized components Ð such as a mechanical analyzer and motorized polarizer Ð to be com- bined.

Fig. 110 Inserting the analyzer

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Leica DMI4000B, DMI3000B, DMI6000B manual Operation Polarization TL, Manual method, Motorized method, Combined methods