Leica DMI3000B, DMI6000B, DMI4000B manual Preparation ¥ Configure the microscope as follows

7. Start-up

¥Using the condenser height adjuster (90.2), ad- just the condenser until the edge of the field diaphragm appears in sharp relief (not S70 condenser).

¥Open the field diaphragm until

it only just disappears from the field of view (91d).

Leica DMI4000 B and Leica DMI6000 B:

¥Select an objective with moderate magnifica- tion (10xÐ20x).

¥Activate the transmitted-light axis with the TL/IL button (84.1).

¥Press the BF button to activate the bright field contrast method (one of the variable function buttons on the stand).

¥Place a specimen on the stage.

¥Focus the specimen using the SmartMove or the focus wheels.

¥Adjust the light intensity with the INT buttons (84.3).

¥Close the field diaphragm with the FD button (84.4) or manually until the edge of the dia- phragm appears in the field of view.

¥Using the condenser height adjuster (90.2), adjust the condenser until the edge of the field diaphragm appears in sharp relief (not S70 condenser).

¥Open the field diaphragm just enough for it to disappear from the field of view (91d).

Note:

The condenser height setting is dependent on the thickness of the specimen and may require adjustment for each new specimen.

Koehler illumination (not for S70 condenser)

Suitable values for the motorized aperture dia- phragm and motorized field diaphragm have been preset for each objective (Leica DMI4000 B and Leica DMI6000 B). The condenser has also been centered at the factory.

However, it may be necessary to readjust the condenser in some cases. Therefore, check the condenser centering.

The following procedure is provided for the transmitted light-bright field illumination.

All required functions can be executed at the touch of a button with the Leica DMI6000 elec- tronic microscope. (See Chapter 8, Operation).

Preparation:

¥Configure the microscope as follows:

Set up the illumination, condenser, objectives and eyepieces correctly. (Please ensure that the objectives are properly screwed in and check the eyepiece settings.)

¥Switch the microscope on and wait for the ini- tialization phase to complete (automatic func- tions only).

¥You will need either an empty Petri dish (pref- erably with a glass bottom) with a marking in the middle or a stained specimen on a slide with a coverslip.

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