7. Start-up

¥Focus on the specimen.

¥Focus the ring structure (95a) by loosening the clamping ring (93.2) somewhat and moving the eyelens (93.1), or focus the Bertrand lens (94.2).

¥Retighten the clamping ring.

Leica DMI3000 B:

¥Select the light ring for the active objective on the condenser.

Leica DMI4000B and Leica DMI6000B:

¥Press the PH (phase contrast) button (one of the variable function buttons behind the focus wheels). The light ring will be selected in the condenser.

¥If the light ring and the phase ring are not shown as arranged in Fig. 95c, the light ring must be centered.

¥Insert the centering keys into the openings provided on both sides of the condenser (90.3).

¥Turn the centering keys until the dark ring (phase ring in the objective) is congruent with the slightly narrower bright ring (light ring in condenser) (95 c).

Caution!

The centering keys must be removed from the centering openings before changing ob- jectives. They may block the condenser.

¥Repeat the process for all additional phase contrast objectives.

¥Remove the centering keys after centering.

Fig. 96 Condenser with motorized polarizer

1Centering key for polarizer

1

Fig. 97 Condenser centering

1Centering openings

2Height adjuster

3Prism and phase ring centering

2

1

2

1

 

 

 

 

 

 

 

 

 

 

 

 

3

3

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Image 74
Leica DMI4000B, DMI3000B, DMI6000B manual Start-up