Leica DMI3000B, DMI6000B, DMI4000B manual Start-up

7. Start-up

Integrated phase contrast with bright field objectives via front slider

When choosing an objective suitable for phase contrast, the appropriate light ring is selected automatically when using a motorized con- denser. Otherwise, select the light ring manually.

Centering the phase rings is not required for ob- jectives with eyepoint A. Checking the position of the phase rings is essential only when using objectives with eyepoint C.

(For the eyepoint of your objective, please refer to the included objective list or the engraving on the objective itself.)

¥Move the front slider with the phase rings into the beam path.

Leica DMI3000 B:

¥ Set the condenser to the bright field position.

Leica DMI4000 B and Leica DMI6000 B:

¥Press the BF (bright field) button (one of the variable function buttons on the stand).

¥Select the objective with the lowest magnifi- cation.

¥Focus on the specimen.

¥Select the objective with the lowest magnifi- cation and eyepoint C.

Leica DMI3000 B:

¥Select the light ring for your current objective on the condenser.

Leica DMI4000 B and Leica DMI6000 B:

¥Press the PH (phase contrast) button (one of the variable function buttons behind the focus wheels). The light ring will be selected in the condenser.

¥Slide the front slider with the phase rings to position C (A and C refer to the eyepoint of the objective. For the eyepoint of your objective, please refer to the included objective list or the engraving on the objective itself.)

¥Instead of an eyepiece, place a focusing tel- escope (Fig. 93) in the observation tube or ac- tivate the Bertrand lens (pull rod (94.1) on tube).

¥Focus the ring structure (95a) by loosening the clamping ring (93.2) somewhat and moving the eyelens (93.1), or focus the Bertrand lens (94.2).

¥Retighten the clamping ring.

¥If the light ring and the phase ring are not shown as arranged in Fig. 95c, the light ring must be centered.

¥Insert the centering key in the opening pro- vided on the front slider

¥Turn the centering keys until the dark ring (phase ring in the objective) is congruent with the slightly narrower bright ring (light ring in condenser) (95 c).

¥Remove the centering keys after centering.

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