Leica DMI3000B, DMI6000B, DMI4000B manual Operation Phase Contrast TL Integrated phase contrast, see

8. Operation

8.2.2Phase Contrast (TL)

(integrated phase contrast, see 8.2.6)

Leica DMI3000 B:

¥Select a phase contrast objective.

¥Select the suitable light ring on the con- denser.

¥Open the aperture of the condenser com- pletely.

¥Remove all other optical components such as analyzers, polarizers or IC prisms from the beam path.

¥Insert a phase contrast specimen.

¥Set the brightness at the light potentiometer

¥Focus the image with the focus wheels.

Leica DMI4000 B and Leica DMI6000 B:

¥Use the TL/IL function button to switch to transmitted light (TL).

¥Select the PH (phase contrast) contrast method

by pressing the variable button PH. Alternatively: press the variable button

CHANGE TL .

(For details on button assignments, please see the identification sheet.)

PH will appear on the LeicaScreen. Motorized condensers will now switch to the correct light ring. Coded condensers must be switched manually.

¥Insert a transmitted light specimen.

¥Rotate an appropriate objective into place. Objectives that are suitable for phase contrast are engraved with PH.

¥Focus the image with the knob on the SmartMove or the focusing wheel and adjust the intensity with the INT function buttons.

Note:

When selecting the phase contrast method, the aperture diaphragm is opened fully and can not be adjusted.

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