To view the effects of any changes that have been made and compare them with the original, use the Processed and Natural tabs in the preview area to switch back and forth between the processed and original image. The image produced when you click the Scan button to open the image in the host application will be the same as the image in the Processed tab.

The Processed /Natural Tabs

When using Nikon Scan as a stand-alone application, you can use the Ctrl-T (Windows) or -T (Macintosh) keyboard shortcut to switch between the Processed and Natural tabs.

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Nikon LS4000 user manual