Chapter 3 Peak Detection and Labeling

3.2 Peak Detection

This section includes:

•Strategy for Mariner peak detection

•Strategy for Voyager peak detection

•Setting peak detection parameters

•Peak detection parameter descriptions

•Charge state determination and examples

3.2.1Strategy for Mariner Peak Detection

 

This section gives some quick suggestions on how to

 

approach Mariner peak detection. For details on peak

 

detection, see Section 3.2.3, Setting Peak Detection

3

Parameters.

Default peak detection values are listed in Section 3.7, Default

 

Peak Detection Settings.

 

Strategy When detecting peaks in Mariner data:

 

1. Open the data file and observe the effects of the default

 

peak detection settings.

 

If you are analyzing resolved isotopes, default settings

 

should yield acceptable peak detection. The default

 

resolution setting of 5,000 is optimized for masses

 

below 3,000 Da.

 

2. If you are analyzing proteins, decrease the Mass

 

Resolution setting.

3-6 Applied Biosystems