Chapter 5 Examining Spectrum Data

11.Click OK.

The result is displayed in the Output window and the zero-charge spectrum is displayed with a DECONV trace label, if selected.

Converting to zero-charge

NOTE: The numerical result displayed in the output window generally is more accurate than the computer-generated spectrum.

12.To return to the original spectrum, click in the toolbar. The number of the original spectrum is displayed in the Select Spectrum dialog box.

13.Click OK.

To convert to a zero-charge spectrum:

1.Activate the Spectrum window.

2.Select Duplicate Active Trace from the Display menu to keep the original data displayed after processing.

3.From the Process menu, select Multiple Charge, then select Convert to Zero-Charge Spectrum.

The Zero-Charge Spectrum Conversion dialog box (Figure 5-12 on page 5-41)is displayed.

4.Specify the Input Spectrum m/z range to include in the calculation by doing one of the following:

•Type in From and To values

•Right-click-drag over a region of the trace that includes the m/z range of interest

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5-40 Applied Biosystems