Chapter 8 Viewing Voyager PSD Data

 

 

NOTE: This selection determines the mass type specified

 

 

for the reference masses in the calibration reference file.

 

 

Use a calibration reference (.REF) file that specifies the

 

 

peak type for reference masses as Resolved Isotope

 

 

Mass (even if they are not resolved isotopes). The

 

 

calibration routine checks peak width to determine if a

 

 

peak matches a Resolved Isotope Mass or an Average

 

 

mass. If narrow peaks are specified as Average

 

 

Masses in the calibration reference file, the software

 

 

mistakes these narrow peaks as isotopically resolved

 

 

and ignores the reference mass. For more information

 

 

on creating calibration reference files, see

 

 

Section 5.3.3, Creating or Modifying a

 

 

Calibration Reference File (.REF).

 

 

 

 

4. Click Induce Fragmentation.

8

 

Results are listed in the ions table, and fragment ions

 

are labeled if they are present in the spectrum.

 

5. Click Create Reference File.

 

 

A Save As dialog box is displayed.

 

6. Name and save the calibration reference file.

 

 

 

 

 

 

Hint: PSD calibration reference files are named with an

 

 

.REF extension. When you create PSD calibration

 

 

reference files, include a _PSD suffix when you name

 

 

files to help you distinguish them from non-PSD

 

 

calibration reference files.

 

 

 

 

 

 

 

 

Hint: Calibration reference files are ASCII text files.

 

 

You can delete unwanted fragment ion entries using

 

 

Microsoft Notepad.

 

 

 

8-22 Applied Biosystems