Chapter 4 Examining Chromatogram Data

4.8Using UV Trace Offset (Mariner Data Only)

To align a UV trace with a chromatogram trace:

1.Display the chromatogram and UV traces of interest.

2.Display the x-axis in retention time for each trace by selecting Traces from the Display menu, selecting X Axis In, then selecting Time.

3.Set the Replace Mode to Add a New Trace. For information see “Setting the Replace mode” on page 2-17.

4.From the Process menu, select Realign UV Trace.

The UV Trace Offset dialog box is displayed (Figure 4-13).

4

Figure 4-13 UV Trace Offset Dialog Box

5. Enter the retention times as follows.

 

 

Click the Original UV Peak Value (min) text box, then

 

right-click-drag over the peak to offset in the UV trace.

 

Select the Aligned UV Peak To (min) text box, then

 

right-click-drag over the peak to align with in the

 

chromatogram trace.

Hint: You can type retention times in the UV Trace Offset dialog box. Use an X data cursor to read the retention time of the peak. If you enter spectrum numbers by mistake, the peak does not align properly.

4-30 Applied Biosystems