Chapter 5 Examining Spectrum Data
5.3.5 Hints for Calibrating Mariner Data
Ensuring that masses match during calibration
Mariner TOF Analyzer parameters affect flight times of ions. If you acquired Mariner data using different TOF Analyzer parameters, and did not calibrate the data in the Instrument Control Panel to compensate for the altered parameters, masses in the data file may be significantly different from the reference masses. For information on calibrating in the Instrument Control Panel, see the Mariner Workstation User’s Guide.
Before performing automatic matching, set the Mass Tolerance and Minimum Intensity appropriately to match the expected reference masses. After automatic matching, check the list of matched peaks to make sure the correct reference masses and peak masses are selected.
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