Chapter 5 Examining Spectrum Data
CAUTION
The software allows you to add multiple items with the same m/z value to the reference file if any other attribute of the reference compound is different (for example, charge state or name). Each mass in the list is considered during calibration. If the mass list contains duplicate entries, the calibration may return an invalid number of matches.
6.To modify an entry, click the reference mass in the list to select it, modify the entry as needed, then click Update. The modified entry is saved in the .REF file.
7.Click Save.
8.In the Save As dialog box, select a location and type a name for the file, then click OK.
The name of the reference file used for mass calibration is stored in the .DAT file and is displayed in the Calibration Reference File field when you open the Manual Calibration dialog box again.
NOTE: Only the name and path of the reference file are stored in the .DAT file. Contents of the file are not stored.
Hint: You can also modify a calibration reference file when you manually select reference masses during calibration. See step 8 on page
To add or delete information in a calibration reference file:
1. | From the Process menu, select Mass Calibration, then |
5 | select Edit/Create Reference File. |
The Edit/Create Reference Peak Information dialog box | |
2. | Click Browse, then select a calibration reference file. |