Voyager Data Examples

7.2 Voyager Data Examples

This section includes:

• Detecting and labeling partially resolved peaks

• Processing before calibrating to optimize mass accuracy

• Detecting peaks from complex digests

7.2.1 Detecting and Labeling

7

Partially Resolved Peaks

 

 

If peaks are not If peaks are partially resolved and the peaks of interest are not

 

 

labeled

labeled, you can adjust the following peak detection

 

 

parameters:

 

 

 

 

 

 

If...

 

Adjust the following...

 

 

 

 

 

 

 

Peaks represent two

 

In the Peak Label dialog box, select Allow Overlapping

 

 

compounds, and you

 

Peak Labels.

 

 

want both peaks labeled

 

OR

 

 

 

 

 

 

 

 

On the Basic Settings tab in Peak Detection, set %Max

 

 

 

 

Peak Area to 0, then adjust the %Base Peak Intensity

 

 

 

 

until peaks are detected.

 

 

 

 

OR

 

 

 

 

On the Peak Processing tab in Peak Detection, change

 

 

 

 

the default Integration Baseline Setting from

 

 

 

 

Valley-to-Valley to Valley-to-Baseline.

 

 

 

 

 

 

 

Peaks represent partially

 

On the Basic Settings tab in Peak Detection, decrease

 

 

resolved isotopes, and

 

the Mass Resolution setting until the isotopic envelope is

 

 

you want to label and

 

detected.

 

 

detect the average mass

 

 

 

 

 

 

 

 

 

 

Figure 7-9shows a spectrum with partially resolved peaks that

 

 

represent two compounds.

 

Data Explorer™ Software User’s Guide

7-11