Chapter 8 Viewing Voyager PSD Data

8.3.2 Calibrating

This section includes:

Calibrating

Matching peaks automatically

Selecting peaks manually

Solving and plotting

Applying new constants to the data file

Selecting calibration peaks for optimum mass accuracy

Calibrating To calibrate a PSD spectrum:

1.Click the Spectrum window to activate it. Select the spectrum of interest.

NOTE: You cannot calibrate a result spectrum or a composite spectrum you have accessed from the

8Processing History command on the Display menu. For more information, see Section 2.4.7, Recalling and Rearranging Traces (Processing History).

2.From the Peaks menu, select Peak Label, then select the Mass Label Type (peak apex or peak centroid) to use for calibration. Click OK.

3.From the Process menu, select Mass Calibration, then select PSD Calibration.

The PSD Processing dialog box is displayed with the Calibration tab selected (Figure 8-5).

Hint: You can also access this dialog box by selecting PSD Processing from the Process menu, then clicking the Calibration tab.

8-12 Applied Biosystems