Chapter 8 Viewing Voyager PSD Data

NOTE: If the calibration reference file is stored on a network drive, an error message may display when you select the calibration file when performing a calibration. If an error message is displayed, copy the file to a local drive on your computer using Windows NT Explorer.

 

5. Enter Reference Matching Criteria:

 

Minimum Intensity— Peaks must be above this

 

intensity to be considered a match. Select the unit

 

for Minimum Intensity, % Relative Intensity or

 

% Relative Area.

 

Mass Tolerance— Peaks must be within this

 

tolerance of the theoretical mass to be considered

 

 

a match. Select the unit, m/z or ppm.

8

6. Select the Peak Weighting Factor. If the calibration

includes more than two points, you can apply the following

weighting factors to fit points to the curve:

 

None— All peaks weighted equally

 

Inverse Peak Width— Narrower peaks are

 

weighted more than broader peaks

 

 

Height— More intense peaks are weighted more

 

than less intense peaks

 

7. Match observed peaks in the spectrum with reference

 

masses in the calibration reference file using one of the

 

following procedures:

 

“Matching peaks automatically” described below

 

“Selecting peaks manually” on page 8-16

8-14 Applied Biosystems