Calibrating a PSD Spectrum

Column header buttons

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Figure 8-5 PSD Processing Dialog Box with

Calibration Tab Displayed

4.Select a PSD Calibration Reference File that you generated as described in Section 8.3.4, Creating PSD Calibration Reference (.REF) Files. A calibration reference file called Angiotensin_Fragments.REF is provided with the software.

NOTE: Use a calibration reference (.REF) file that specifies the peak type for reference masses as Resolved Isotope Mass (even if they are not resolved isotopes). The calibration routine checks peak width to determine if a peak matches a Resolved Isotope Mass or an Average mass. If narrow peaks are specified as Average Masses in the calibration reference file, the software mistakes these narrow peaks as isotopically resolved and ignores the reference mass. For more information on creating calibration reference files, see Section 5.3.3, Creating or Modifying a

Calibration Reference File (.REF).

Data Explorer™ Software User’s Guide

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