Appendix C Data Explorer Toolbox (Visual Basic Macros)

C.7 Using MS Fit/MS Tag Toolbox
Preparing data before accessing

C

Running

 

MS Fit/MS tag

 

 

Use the MS Fit/MS Tag toolbox when analyzing protein digest, peptide, or peptide fragment spectra to perform a protein database search.

Before using the MS Fit/MS Tag toolbox:

1.In the Data Explorer software, smooth the data, perform a baseline correction, then deisotope.

2.If needed, use Advanced Peak Detection parameters to adjust peak detection ranges and thresholds to screen out noise in low-mass regions and to detect peaks in high-mass regions.

3.Set the Set the %Base Peak Intensity or %Max Peak Area in peak detection until the unwanted noise peaks are removed from the Spec Peak list.

To run the MS Fit/MS Tag macro:

1.In the Toolbox Palette dialog box, click MS Fit/MS Tag.

Figure 3-15 MS Fit Dialog BoxMS Tag Dialog Box

C-18 Applied Biosystems