Appendix C Data Explorer Toolbox (Visual Basic Macros)
C.7 Using MS Fit/MS Tag ToolboxC | Running |
| MS Fit/MS tag |
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Use the MS Fit/MS Tag toolbox when analyzing protein digest, peptide, or peptide fragment spectra to perform a protein database search.
Before using the MS Fit/MS Tag toolbox:
1.In the Data Explorer software, smooth the data, perform a baseline correction, then deisotope.
2.If needed, use Advanced Peak Detection parameters to adjust peak detection ranges and thresholds to screen out noise in
3.Set the Set the %Base Peak Intensity or %Max Peak Area in peak detection until the unwanted noise peaks are removed from the Spec Peak list.
To run the MS Fit/MS Tag macro:
1.In the Toolbox Palette dialog box, click MS Fit/MS Tag.
Figure