Peak Labeling

3.5.1 Charge State Labels

Charge state labels

Requirements

A unique feature of the Data Explorer software is the ability to label multiply charged isotope peaks with their charge state.

In general, the observed spacing between isotopes is determined by the charge state of the detected ion. Spacing between isotope peaks is narrower at higher charge states. For more information on isotopes, see Appendix B, Overview of Isotopes.

To determine charge state, the software uses the following charge state determination parameters:

•Max Charge State

•Max Isotope #

•Min and Max Intensity

For a description of these parameters, see “Advanced

Settings (spectrum data only)” on page 3-28and “Charge 3 State Determination and Examples” on page 3-32.

NOTE: If Charge State Determination parameters are set incorrectly, the charge state is determined incorrectly. For more information, see “Charge state parameter examples” on page 3-32and “Charge State and Isotope Determination Troubleshooting—Mariner Only” on page 9-18.

To label isotope peaks with charge states, the following must be true:

•Mass must be within the range in which the system can resolve the isotope peaks:

•<4,000 Da on a Mariner system

•<5,000 Da on a Voyager-DE PRO system

•<6,000 Da on a Voyager-DE STR system

Data Explorer™ Software User’s Guide

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