Chapter 7 Data Explorer Examples
7.1.2Deconvoluting and Evaluating Unresolved Chromatographic Peaks
Overview
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Creating a combined spectrumYou can use the Data Explorer software to deconvolute chromatographic peaks and obtain masses for each component by:
•Creating a combined spectrum to identify the coeluting species by mass
•Creating extracted ion chromatograms for the masses, and comparing the extracted ion chromatograms to the original TIC containing the unresolved peaks
You can then create a combined spectrum from each extracted ion chromatogram to evaluate the spectral data.
The following example illustrates how to deconvolute two unresolved chromatographic peaks in a tryptic digest of cytochrome c:
1.Display the data file.
2.In the Chromatogram window, zoom in on the unresolved peaks (Figure
Figure
3.In the Chromatogram window,