Chapter 6 Using Tools and Applications

6

Figure 6-15 Ion Fragmentation Calculator Dialog Box

4.Type or copy the amino acid or residue sequence of interest. Use single-letter codes.

Sequence codes are case-sensitive. Click the User-Defined Amino Acids button to display the list of allowed residues and their associated codes.

5.Select the N-terminus and C-terminus options for the sequence.

6.Specify the cysteine modification, and specify the mass type (average or monoisotopic) for the calculated fragment ion masses.

6-26 Applied Biosystems