HP Data Explorer 4 B.2 Monoisotopic, and Average Masses

Appendix B Overview of Isotopes

B.2 Monoisotopicand Average Masses

B

When isotopes are clearly resolved (Figure B-6), the monoisotopic mass is used for mass labeling, and corresponds to the lowest mass peak in the cluster.

Monoisotopic mass corresponds to lowest mass peak

Figure B-6 Monoisotopic Mass

When isotopes are not resolved (Figure B-7), the average mass is used for mass labeling, and corresponds to the centroid of the unresolved peak cluster (weighted average of all isotope peaks in the cluster).

B-6 Applied Biosystems