Using the Ladder Sequencing Toolbox
7.Under Annotate Spectrum, select the types of labels you want displayed:
•Reference Mass (*) Mass of the reference peak against which the current peak is compared.
•Mass Difference Difference between the current peak and the reference, preceded by a minus sign
•Peak Mass Mass of the current peak, preceded by an equal sign (=).
8.Click Label Peaks.
If Peptide is selected, the software:
•Examines the spectrum in 55 Da increments
and selects the most intense ion in the range.
(The 55 Da increment is used because it is C less than the smallest mass difference related
to a residue.)
•Labels mass differences (plus or minus the specified Tolerance) that correspond to amino acids.
•Labels the reference peak from which the mass difference was derived with an asterisk (*).
•Applies the additional labels you selected under Annotate Spectrum.
Data Explorer™ Software User’s Guide |