Chapter 7 Data Explorer Examples

7

 

 

Figure 7-22 Deisotoping Dialog Box

 

10.

For this example spectrum, specify H for Adduct and

 

 

C6H5NO for Generic Formula.

 

11.

Click OK. For more information on deisotoping, see

 

 

Section 3.4, Deisotoping a Spectrum.

Increasing

12.

In the Basic Settings tab in Global Thresholds (see

detection

 

Figure 7-20 on page 7-20),increase %Max Peak Area

thresholds

 

to a setting between 0.1 and 1% until the peaks of

 

 

interest are detected and noise is screened out.

Figure 7-23shows the trace after deisotoping. Note that many fewer peaks are detected. By further fine-tuning %Max Peak Area, you can screen out additional noise peaks.

Figure 7-23 Digest After Noise Filtering, Deisotoping,

and Adjusting Detection Thresholds

7-22 Applied Biosystems