Table of Contents

Chapter 7 Data Explorer Examples

7.1

Mariner Data Examples ............................................................

7-2

 

7.1.1

Improving Signal-To-Noise Ratio ............................................

7-2

7.1.2Deconvoluting and Evaluating

 

 

Unresolved Chromatographic Peaks .......................................

7-4

 

7.1.3

Determining if a Peak is Background Noise ............................

7-8

7.2

Voyager Data Examples ..........................................................

7-11

 

7.2.1

Detecting and Labeling Partially Resolved Peaks ..................

7-11

7.2.2Processing Before Calibrating to Optimize Mass Accuracy ...7-14

7.2.3 Detecting Peaks from Complex Digests ................................7-18

Chapter 8 Viewing Voyager PSD Data

8.1

Displaying PSD Data ...............................................................

8-2

8.2

Applying Fragment Labels.........................................................

8-8

8.3

Calibrating a PSD Spectrum .....................................................

8-10

 

8.3.1

Checking Peak Detection ......................................................

8-11

 

8.3.2

Calibrating ............................................................................

8-12

8.3.3Creating PSD Calibration (.CAL) Files and

 

Applying to Other Data Files .................................................

8-20

8.3.4

Creating PSD Calibration Reference (.REF) Files .................

8-21

8.3.5

Changing the Precursor Mass ...............................................

8-23

Chapter 9 Troubleshooting

9.1

Overview ..............................................................................

9-2

9.2

General Troubleshooting ..........................................................

9-3

9.3

Processing, Tools, and Applications Troubleshooting .......................

9-6

9.4

Calibration Troubleshooting ......................................................

9-10

9.5

Printing Troubleshooting ..........................................................

9-14

9.6

Peak Detection and Labeling Troubleshooting...............................

9-15

viii Applied Biosystems