Adjusting the Baseline

 

 

Parameter

Description/Specifies

 

 

Degree

Determines how closely the calculated baseline fits the

 

data. Valid entries are 0.0 to 1.0.

 

A value closer to 1 fits the baseline more closely to the

 

data, and corrects the midpoint of the noise signal to

 

approximately 0 intensity.

 

A value closer to 0 fits the baseline less closely to the

 

data, and corrects the midpoint of the noise signal to a

 

value greater than 0 intensity (see below).

 

For protein and tryptic digest peptide spectra, use 0 to

 

0.5. If the baseline after correction is too high, increase

 

the Degree value (see below).

Degree=1

Degree=0

Midpoint of noise signal

Midpoint of noise signal

corrected to 0

corrected above 0

A lower Degree setting allows the baseline correction to occur more quickly, and often provides better correction than a higher setting.

NOTE: Flexibility and Degree parameters may require some testing to determine the optimum settings for your data.

 

 

5

4. Click OK.

The baseline is adjusted, and the trace is displayed with a

AdvBC trace label.

Data Explorer™ Software User’s Guide

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