Mass Deconvolution (Mariner Data Only)

5.6Mass Deconvolution (Mariner Data Only)

Overview

Mass Deconvolution

NOTE: Mass deconvolution is not supported for

Mariner DAD data.

NOTE: The Mass Deconvolution software is an option in the Data Explorer software. The Multiple Charge command on the Process menu is dimmed if you have not purchased the option.

You can use the Data Explorer Mass Deconvolution features to generate a theoretical zero-charge spectrum that represents the molecular mass of a protein:

•Mass Deconvolution— Use when you have a spectrum with clearly resolved, multiply charged peaks. You specify the m/z values for the peaks to include. This function requires at least two adjacent peaks within the same charge envelope.

•Convert to Zero-Charge Spectrum— Use when you have a spectrum with overlapping charge envelopes, or a noisy baseline. You specify a mass range for the peaks to include and a mass range for the molecular mass.

To use the Mass Deconvolution function:

1.Click the Spectrum window.

2.Select Duplicate Active Trace from the Display menu to keep the original data displayed after processing.

3. From the Process menu, select Multiple Charge, then

5

select Mass Deconvolution.

 

Data Explorer™ Software User’s Guide

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