HP Data Explorer 4 Creating extracted ion chromatogram

Chapter 7 Data Explorer Examples

7

Figure 7-7 Subtracted Spectrum

The peak at 391 is still present, which indicates one of the following conditions:

•You did not subtract sufficient baseline

•The peak is a coeluting component

Creating extracted ion chromatogram

To evaluate further, create an extracted ion chromatogram for 391 Da as described below and evaluate the signal.

To create the extracted ion chromatogram, right-click-drag over the peak at 391 Da in the spectrum trace.

NOTE: You can also create an extracted ion chromatogram by selecting Trace from the Display menu, selecting Extracted Ion, then entering a mass.

Figure 7-8illustrates the extracted ion chromatogram for mass

391.The signal is relatively consistent, indicating that it is background instead of a component peak.

Figure 7-8 Extracted Ion Chromatogram for Mass 391 Da

7-10 Applied Biosystems