If the Max Isotope # is set too low, the software incorrectly groups peaks into different isotope clusters. In this example with the Max Isotope # set to 2 and Max Charge State set to 4, the software labels all peaks as charge state 3 (Figure 3-10).
Figure 3-10 Max Isotope # Set Too Low, Max Charge
State Set Correctly
3
The effect of setting Max Isotope # too low is apparent when you turn on Monoisotopic peak list filtering. Figure 3-11shows that the software has grouped the peaks into two clusters and has identified two monoisotopic peaks.
Figure 3-11 Max Isotope # Set Too Low, Monoisotopic
Peak List Filtering Turned On
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