Chapter 4 Examining Chromatogram Data

2.Select the method to use based upon the type of data you are examining, then enter the associated value displayed for the method you select:

 

 

Type of Data

Suggested

Description

 

 

Method

 

 

 

 

 

 

 

 

 

 

 

Higher resolution

Noise Filter

Specify a Correlation Factor of 0 to 1.0.

 

 

data

(NF)

Settings from 0.5 to 0.7 yield acceptable

 

 

 

 

 

 

 

 

results for most data. A setting close to 1.0

 

 

 

(May affect

yields a higher degree of noise reduction.

 

 

 

 

 

 

 

peak

If applying the Noise Filter with a certain

 

 

 

resolution.)

Correlation Factor does not yield the

 

 

 

 

necessary noise removal, return to the original

 

 

 

 

trace (see “Returning to the original trace” on

 

 

 

 

page 4-4)and apply the Noise Filter again with

 

 

 

 

a higher Correlation Factor setting. Applying

 

 

 

 

the filter more than one time with the same

 

 

 

 

Correlation Factor setting does not improve

 

 

 

 

noise removal.

 

 

 

 

 

 

 

Higher molecular

Gaussian

Specify a Filter Width in data points (odd

 

 

weight data

Smooth

integers only). The maximum number of

 

 

 

(SM)

smoothing points is 2001. Points less than 1

4

 

 

 

Filter Width from the edge of the spectrum are

 

 

(May affect

not smoothed.

 

 

 

 

 

 

 

 

 

 

peak

 

 

 

 

resolution.)

 

 

 

 

 

 

 

 

 

 

 

4-18 Applied Biosystems